TITLE	In vitro polysome translation analysis of heat shock proteins in higher plants
AUTHOR	Hwa Dai Institute of Botany, Academia Sinica, Taipei, Taiwan 11529, Republic of China Yih-Shan Lo Institute of Botany, Academia Sinica, Taipei, Taiwan 11529, Republic of China Yaw-Huei Lin Institute of Botany, Academia Sinica, Taipei, Taiwan 11529, Republic of China Manfred Ruddat Department of Ecology and Evolution and 3 Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Ill. 60637, USA Kwen-Sheng Chiang
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ABSTRACT	We used a simple and highly reproducible method for the analysis of heat shock proteins in rice (Oriza sativa), maize (Zea mays), and mung bean (Vigna radiata). Cytosolic polysomes, isolated from as little as 1_4 grams of seedling biomass, were in vitro translated with the wheat germ system. SDS-PAGE or two Dimensional-PAGE produced excellent separation of the translation products. The active, translating mRNA induced by heat shock was shown by this easy and reproducible method.
KEYWORD	In vitro polysome translation; Heat shock proteins;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 37 Number 4 October 1996, page 261-264, 4 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China