TITLE	The isolation and characterization of Chaperonin 60 from sweet potato roots -- Involvement of the chaperonins in starch biosynthesis
AUTHOR	Shih-Chung Chang Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan Pei-Chun Lin Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan Han-Min Chen Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan Jiann-Shing Wu Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan Rong-Huay Juang Department of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan
FULL TEXT	[in HTML format] [in PDF format]
ABSTRACT	Molecular chaperonins cpn60a and cpn60b were isolated from sweet potato roots and were characterized as homologous polymers. However, their immunochemical and biochemical properties were divergent. The two chaperonins as well as starch phosphorylase appeared concurrently during the starch-accumulating period and were localized in the starch granule by histochemical methods. Notably, these proteins increased substantially in cells around the anomalous cambium when sweet potato initiated starch biosynthesis. Observations in this study indicated that the chaperonins might be involved in the biosynthesis of starch in sweet potato roots.
KEYWORD	Amyloplast; Anomalous cambium; Chaperonins; Histochemical methods; Ipomoea batatas; Starch biosynthesis; Starch phosphorylase;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 2 April 2000, page 105-111, 7 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China