TITLE	RFLP and inheritance patterns of chloroplast DNA in intergeneric hybrids of Phalaenopsis and Doritis
AUTHOR	Song-Bin Chang Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China Wen-Huei Chen Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China Hong-Hwa Chen Department of Biology, and 3Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan 701, Republic of China Yang-Ming Fu Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China Yih-Shyan Lin Department of Horticulture, Taiwan Sugar Research Institute, Tainan, Taiwan 702, Republic of China
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ABSTRACT	The mode of inheritance of chloroplasts was analyzed using restriction fragment length polymorphism (RFLP) in both interspecific hybrids of Phalaenopsis and intergeneric hybrids of Phalaenopsis and Doritis. Chloroplast DNA digested with Dra I followed by hybridization with an rbcL probe revealed that Phalaenopsis amabilis, Phalaenopsis aphrodite, and Phalaenopsis stuartiana, which belong to the taxonomic section PHALAENOPSIS, have the same size 2.0-kb fragment. Both Phalaenopsis mannii and Phalaenopsis amboinensis have a 2.3-kb fragment, while Doritis pulcherrima has a 3.5-kb fragment. In both interspecific and intergeneric hybrids, maternal inheritance of the chloroplast genome was detected. The hybrids of both reciprocal crosses (A x B and B x A) are registered with the same hybrid names in Sander's List of Orchid Hybrids at the Royal Horticultural Society, despite harboring chloroplast DNA from different parents. These results suggest that the chloroplast DNA can be used as a marker for identification of parentship and for phylogenetic studies of taxonomy.
KEYWORD	cpDNA inheritance pattern; Doritis; Phalaenopsis; RFLP;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 41 Number 3 July 2000, page 219-223, 5 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China