TITLE RAPD variation in relation to population differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis
AUTHOR Shih-Ying Hwang
Graduate Institute of Biotechnology, Chinese Culture University, 55 Hwagan Rd., Yangmingshan, Taipei, Taiwan
Huei-Wen Lin
Graduate Institute of Biotechnology, Chinese Culture University, 55 Hwagan Rd., Yangmingshan, Taipei, Taiwan
Yi-Shou Kuo
Graduate Institute of Biotechnology, Chinese Culture University, 55 Hwagan Rd., Yangmingshan, Taipei, Taiwan
Tsan-Piao Lin
Division of Silviculture, Taiwan Forestry Research Institute, 53 Nanhai Rd., Taipei 100, Taiwan
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ABSTRACT The population differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis based on random amplified polymorphic DNA (RAPD) variation is described. Two populations (Chilanshan and Alishan) of these two Chamaecyparis species are investigated for population differentiation. Shannon's phenotypic index was used to estimate Ho of these two Chamaecyparis species based on RAPD variation. RAPD analysis showed that the Chilanshan population had higher genetic diversity than the Alishan population in both Chamaecyparis species. These results correlated with the large population found in Chilanshan for both species. Based on RAPD analysis, there was 15.13% population differentiation between Chilanshan and Alishan of C. formosensis compared with 14.73% for C. taiwanensis. Higher levels of genetic variation and population differentiation indicated dynamic evolution in these two Chamaecyparis species in Taiwan as revealed by variation at RAPD loci.
KEYWORD Chamaecyparis formosensis; Chamaecyparis taiwanensis; Population differentiation; RAPD;
ARTICLE INFO Botanical Bulletin of Academia Sinica, Volume 42 Number 3 July 2001, page 173-179, 7 pages
PUBLISHER Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China