TITLE	Ultrastructural study and lipid formation of Isochrysis sp. CCMP1324
AUTHOR	Ching-Piao Liu Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan, Republic of China Liang-Ping Lin* Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei 106, Taiwan, Republic of China
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ABSTRACT	This study investigates methods for extracting lipids from microalgae and analyzes the effects of culture media as well as culture conditions on PUFA yields and total fatty acid contents. Experimental results of an optimal culturing of Isochrysis spp. were based on a 3.2% salinity culture medium. These microalgae were cultured in a 1-2 L Roux's flat-flask and a 5 L jar fermentor. The optimum culture temperature and initial pH for DHA production were 25°C and 8.0, respectively. Pigments included chlorophylls a and c. The DHA yield increased with cultivation time until the eighth day. Optimum DHA amounts in the cells were reached under aeration with 10% CO2 and with continuous illumination of 10 klux. The biomass dry weight reached 4 g per liter of culture, and the DHA production reached 16 mg per liter of culture. Lipid bodies in Isochrysis spp. and related genera were observed during culture by light and transmission electron microscopy; 0.5~3.0 µm sized lipid bodies were confirmed by staining with Sudan Black B in cells from log stage to stationary stage cultures. These results demonstrated that DHA-containing lipid bodies in cells can be produced and accumulated in marine Isochrysis spp.
KEYWORD	Docosahexaenoic acid; Isochrysis sp.; Lipid formation; Polyunsaturated fatty acids (PUFA); Ultrastructure;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 42 Number 3 July 2001, page 207-214, 8 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China