TITLE	Fine mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice
AUTHOR	Jingyu Huang The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China Jun Hu The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China Xin Xu College of Life Sciences and Chemistry, South-Central University for Ethnic Communities, Wuhan, 430074, P. R. China Shaoqing Li The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China Ping Yi The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China Daichang Yang Ventria Bioscience 4110 Freeway, Sacramento, CA 95834, USA Fugang Ren The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China Xuequn Liu The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China Yingguo Zhu The Key Laboratory of Ministry of Education for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, P. R. China
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ABSTRACT	Bulked segregant analysis (BSA) of a BC1 population derived from congguang 41A/MiYang 23//congguang 41B was used to map the nuclear fertility restorer gene Rf5 for HongLian (HL) cytoplasmic male sterility. The parents and two bulks representing extremely fertile and sterile plants, respectively, were screened for polymorphism with 20 microsatellite primer pairs on chromosome 10, chosen on the basis of previous research. MRG4456 is linked to the fertility restorer gene Rf5 at a distance of 1.57 cM, and another newly developed microsatellite primer, HL01, was locked at a distance of 0.63 cM to Rf5. Closely linked DNA markers will facilitate not only breeding but also the purity management of hybrid seeds. Concrete steps for developing new microsatellite markers using the rice whole genomic sequence database are also described.
KEYWORD	Fertility restorer gene; Marker-assisted selection; Microsatellite marker; Oryza sativa L;
ARTICLE INFO	Botanical Bulletin of Academia Sinica, Volume 44 Number 4 October 2003, page 285-289, 5 pages
PUBLISHER	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China